Mare´s milk as a source of ACE-inhibitory peptides for human nutrition

Summary Within this study the whey proteins±-lactalbumin,²-lactoglobulin 1 and 2 and the caseins±S1-casein,±S2-casein,²-casein andº-casein of mare´s milk have been processed in silico with the enzymes pepsin (E.C. 3.4.23.1), trypsin (E.C. 3.4.21.4) and chymotrypsinA (E.C. 3.4.21.1) using the programme BIOPEP analyzing the ACE-inhibitory peptides, which evolvean antihypertensive effect. At the same time the effect of genetically variatedversions of horse milk proteins on the occurrence of ACE-inhibitory peptides waschecked. The in silico analysis of the included milk protein sequences resulted in 17 different bioactive peptides with ACE-inhibitory function. The Peptide IVR out of±S2-Kasein having a very low IC50-value of 0,81 ¼M...